Explant type and different concentrations of salts in MS medium in vitro establishment of Aniba canelilla

  • Santiago Linorio Ferreyra Ramos Faculty of Agricultural Sciences, Federal University of Amazonas
  • Maria Teresa Gomes Lopes Faculty of Agricultural Sciences, Federal University of Amazonas
  • Paulo de Tarso Barbosa Sampaio National Institute for Amazonian Research, Department of Tropical Forestry
  • Edvan Alves Chagas Embrapa, Embrapa CPAFRR.
  • Moacir Pasqual Federal University of Lavras, Department of Agriculture. DEPARTMENT OF AGRICULTURE
  • Perla Pimentel da Silva Postgraduate in Nuclear Energy in Agriculture. CENA / USP.

Abstract

The peel - precious ( Aniba canelilla ) is a plant species native to the Amazon region , with economic potential in silvicultural terms , the production of perfumes and cosmetics . However , one limitation to the domestication of the species is the absence of propagation methods . The objective of this study was to evaluate the effect of explant type and different salt concentrations of MS medium in their establishment in vitro . Shoot tips ( 1.5 cm ) and nodal segments were used with two gems, isolated from bark - precious seedlings maintained in the greenhouse . The treatments consisted of two types of explants and six concentrations of salts in MS medium plus 15 or 30 g L- 1 sucrose and disinfectants . It was found that the nodal segment is less sensitive to treatments performed disinfestation and decontamination showed higher successful in survival and in vitro establishment of shell - precious.

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Author Biographies

Santiago Linorio Ferreyra Ramos, Faculty of Agricultural Sciences, Federal University of Amazonas
Doctorate in progress in Agronomy (Plant Breeding and Genetics)
Maria Teresa Gomes Lopes, Faculty of Agricultural Sciences, Federal University of Amazonas

Graduated in Agronomy from the Federal University of Viçosa (1996), MSc in Genetics and Breeding, Federal University of Viçosa (1998) and Ph.D. in Agronomy (Plant Breeding and Genetics) from the University of São Paulo (2003). He is currently assistant professor at the Federal University of Amazonas. Works in the area of genetics and plant breeding.

Paulo de Tarso Barbosa Sampaio, National Institute for Amazonian Research, Department of Tropical Forestry
He holds a Ph.D. in Forestry Engineering from the Federal University of Paraná (1996). He is currently Head Researcher of the National Institute of Amazonian Research and professor at the University of the State of Amazonas. Has experience in the area of Forest Resources and Forest Engineering, with emphasis on Forestry. Acting on the following topics: oocarpa Pinus, Pinus caribaea var hondurensis, Pinus maximinoi, individual selection, selection and combined index selection for multi-purpose.
Edvan Alves Chagas, Embrapa, Embrapa CPAFRR.

Agronomist graduated from the Federal University of Pelotas (2001), M.Sc. in Plant Science (Tissue Culture) by Federal University of Lavras (2003) and PhD in Plant Production (Tropical Fruits) Universidade Estadual Paulista (FCAV / UNESP) in 2005. Since 2009, he joined the research staff of the Brazilian Agricultural Research Corporation (EMBRAPA).

Moacir Pasqual, Federal University of Lavras, Department of Agriculture. DEPARTMENT OF AGRICULTURE
Graduated in Agronomy from the Federal University of Pelotas (1971), master's degree in Agronomy (Plant Science) from the Federal University of Lavras (1976) and Ph.D. in Genetics and Plant Breeding the School of Agriculture Luiz de Queiroz - University of São Paulo (1985 ). Was Dean of Student, Community and Cultural Affairs UFLA; Member of the Committee of Agronomy CNPq. Was awarded the Linda Caldas Senior Category Award by the Brazilian Association of Tissue Culture and Plant-ABCTP with Secundino Antonio Commandery of St. Joseph by the State of Minas Gerais. He is currently coordinator of Agricultural Sciences area I Coordination of Improvement of Higher Education Personnel (CAPES), position he holds since 2008, and full professor at the Federal University of Lavras (UFLA).
Perla Pimentel da Silva, Postgraduate in Nuclear Energy in Agriculture. CENA / USP.

Doctoral PG Sciences
(NUCLEAR ENERGY IN AGRICULTURE).
SCENE. Center for Nuclear Energy in Agriculture
Master in Urban Biology.

References

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FERREIRA, M. G. R.; SANTOS, M. R. A.; BRAGADO, A. C. R. Propagação in vitro de cupuaçuzeiro: desinfestação de explantes florais. Saber Científico, v. 2, n. 2, p. 37-44, 2009.

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SAMPAIO, P. T. B. Preciosa Aniba canelilla. In. CLAY, J. W.; SAMPAIO, P. T. B; CLEMENT, C. (Eds.). Biodiversidade Amazônica: Exemplos e estratégias de utilização. 1. ed. Manaus: Instituto Nacional de Pesquisas da Amazônia, 2000. p. 298-305.

SILVA, A. L. L.; FRANCO, E. T. H.; BISOGNIN, D. A.; DORNELLES, E. B.; WALTER, J. M. Efeitos do nitrato de amônia na multiplicação e regeneração de gemas laterais de Dyckia maritima Baker - Bromeliaceae. Revista Brasileira de Agrociência, v. 11, n. 3, p. 369-371, 2005.

TEIXEIRA, J. B. Limitações ao processo de cultivo in vitro de espécies lenhosas. Brasília: Embrapa-Recursos Genéticos e Biotecnologia, 2001. Disponível em: <http://www.redbio.org>. Acesso em: 26 nov. 2011.

Published
2013-12-05
Issue
Vol 56 No 4 (2013): RCA
Section
Scientific Note

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