Genetic variability of isolates of Rhizoctonia solani, the shoots analyzed by RAPD markers
Abstract
Rhizoctonia solani is a cosmopolitan fungus with large number of hosts and cause important diseases in many cultivated plants around the world. Different techniques have been used in order to study the genetic diversity of R. solani. Among them, the DNA markers RAPD (Random Amplified Polymorphic DNA) are tools to characterize and evaluate germplasm quickly and efficiently. Because of the variability in symptoms produced by this pathogen, the objective of this study is to evaluate the genetic variability of 13 isolates of R. solani that induce, mostly symptoms in shoots of infected plants, using molecular markers DNA, RAPD. Molecular analysis of extracted DNA of isolates with primers OPA1 and OPA2 allowed visualization of 17 polymorphic bands with sizes ranging from 800-1800 bp, generating a 100% polymorphism among the isolates 13
studied. The similarity between the samples, estimated by the Jaccard coefficient was 21.71%, being generated by the UPGMA dendrogram one that allowed grouping the isolates into seven main groups. Based on assessments conducted, it was concluded that there is a large molecular variability
among isolates of R. solani analyzed.
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