Profile of in vitro-grown aloe plants using different concentrations of 6-benzylaminopurine

Olival Teixeira Malta; Sthefany Hevhanie  Vila Verde Souza; Mariane  de Jesus da Silva de Carvalho; Eva Maria Rodrigues Costa; Paulo Roberto  Ribeiro Mesquita; Weliton Antônio  Bastos de Almeida

Olival Teixeira Malta Faculdade Maria Milza https://orcid.org/0000-0002-7619-3000
Sthefany Hevhanie Vila Verde Souza Faculdade Maria Milza https://orcid.org/0000-0002-3126-9112
Mariane de Jesus da Silva de Carvalho Faculdade Maria Milza https://orcid.org/0000-0003-0443-6912
Eva Maria Rodrigues Costa Faculdade Maria Milza https://orcid.org/0000-0003-1538-8709
Paulo Roberto Ribeiro Mesquita Faculdade Maria Milza https://orcid.org/0000-0003-3187-3800
Weliton Antônio Bastos de Almeida Centro Universitário Maria Milza https://orcid.org/0000-0002-8941-5936

Keywords: aloe vera, secondary metabolites, active principles

Abstract

Aloe plants have a great therapeutic potential, which is attributed to the several bioactive compounds found in their composition. Therefore, the objective of this work was to evaluate the profile of secondary metabolites and the morphophysiological characteristics of Aloe plants collected at different periods and submitted to different growing conditions. So, axillary buds were collected at two different periods and grown in vitro in MS culture medium containing naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) under controlled conditions for 30 days. Then, the morphophysiological characterization and the analysis of the profile of metabolites produced by the plants were performed (HS-SPME / GC-MS techniques). The results showed that the BAP concentrations did not influence the morphophysiological characteristics of the plants. Almost all plants showed a 100% rooting percentage (RP) when grown in vitro, both with and without BAP in the nutrient medium. Only the plants subjected to BAP concentration of 3 mg L^-1 showed a lower RP. The rise in BAP concentrations also promotes a reduction in the total area of volatile organic compounds (VOCs) in plants. Some compounds (hexanal; 2-hexenal; 1-hexanol; 1-nonanol; 2-decanone; beta-ionone) showed a significant reduction (> 0.10%) in their concentration as the concentration of BAP was incremented in the medium cultivation. Nevertheless, the in vitro cultivated plants showed a greater total area and concentration of VOCs than the in vivo cultivated plants.

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Author Biographies

Olival Teixeira Malta, Faculdade Maria Milza

Degree in Dentistry and Master in Regional Development and Environment.

Sthefany Hevhanie Vila Verde Souza, Faculdade Maria Milza

Undergraduate in the Bachelor's Degree in Physiotherapy at Faculdade Maria Milza (FAMAM) by the Universidade Para Todos Program (PROUNI). Volunteer of PROINC 2019-2020 in the area of Biotechnology Applied to Health with a research line in Tissue Culture. Member of the Plant Biotechnology Applied to Health and Sustainability Research Group at Faculdade Maria Milza (FAMAM).

Mariane de Jesus da Silva de Carvalho, Faculdade Maria Milza

Graduated in Agricultural Engineering from the Federal University of Recôncavo da Bahia (2010), Master in Plant Genetic Resources (2013) and Ph.D. in Agricultural Sciences from the same educational institution (2016). She is currently Permanent Professor of the Professional Master's Program in Regional Development and Environment and the Professional Master's Program in Biotechnology at Faculty Maria Milza - FAMAM and, in addition, Professor of Biostatistics (Graduate Courses in Nursing and Pharmacy) and Statistics ( Undergraduate courses in Accounting and Administration) from the same educational institution. She works in the areas of Plant Genetic Improvement, Plant Genetic Resources, Statistics and Agricultural Experimentation, with an emphasis on experiment planning, statistical data analysis and tissue culture of banana, bromeliads, citrus, cassava, orchids and medicinal plants.

Eva Maria Rodrigues Costa, Faculdade Maria Milza

Graduated in Agricultural Engineering from the Federal University of Piauí (2007), Master in Agronomy (Genetic Plant Breeding) from the Federal Rural University of Pernambuco (2010) and Ph.D. in Genetics and Plant Breeding from the Federal University of Lavras (2013).

Paulo Roberto Ribeiro Mesquita, Faculdade Maria Milza

Bachelor of Chemistry (2010), Master of Chemistry (2012) and Doctor of Chemistry (2016) from the Federal University of Bahia, being approved with distinction. Since the first semester of the undergraduate course (2006), he has been developing research activities at the Laboratory for Research and Development in Chemistry (LPQ-IQ / UFBA), coordinated by Prof. Dr. Jailson Bittencourt de Andrade. He also worked as a researcher at the Agricultural Technological Center of the State of Bahia (CETAB), linked to the Department of Agriculture of the State of Bahia, where he developed research aimed at Bahian agriculture, with an emphasis on the development of methods for analyzing pesticide residues in fruits and in chemical ecology applied to the management of agricultural pests and diseases. He is a professor of the Bachelor of Pharmacy course at the Faculty Maria Milza (FAMAM), where he is also an advisor in the Postgraduate Programs in Regional Development and Environment and in Biotechnology. He also taught in the Pharmacy course at Faculdade Maurício de Nassau. Has experience in Chemistry, working mainly on the following subjects: Environmental Chemistry, Analytical Methods, Chromatography, Mass Spectrometry, SPME, pesticides, extraction and analysis of volatile organic compounds, metabolomics, Leishmaniasis, chemical ecology of insect pests and vectors of diseases, in addition to the development of chemical methods for diagnosing diseases important to agriculture and public health.

Weliton Antônio Bastos de Almeida, Centro Universitário Maria Milza

Graduated in Agronomy from the SCHOOL OF AGRONOMY OF THE FEDERAL UNIVERSITY OF BAHIA (1985), Master in Agricultural Sciences from the Federal University of Bahia (1994) and Doctorate in Plant Science from the University of São Paulo (2002). He is currently Director of Honor at Faculdade Maria Milza and Retired Adjunct Professor at the Federal University of Recôncavo da Bahia. He has experience in Agronomy, with an emphasis on Plant Anatomy and Organography, working mainly on the following topics: biotechnology, in vitro cultivation, micropropagation, tissue culture, plant anatomy and medicinal plants. Collaborator of the Graduate Programs at Faculdade Maria Milza: 1 - Master's Degree in Regional Development and Environment; 2 - Master's Degree in Biotechnology.

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